VWF activity and thrombin generation kinetics in trauma patients versus healthy volunteers*
| Including only samples obtained from trauma patients at 0–2 hours from TOI | Healthy volunteers (n=21 samples) | Trauma (n=22 samples) | P value |
| REAADS activity (%) | 92.0 (71.0–114.0) | 190.0 (132.0–264.0) | <0.001 |
| ADAMTS-13:Ag (µg/mL) | 1.00 (0.89–1.09) | 0.84 (0.51–0.94) | 0.010 |
| VWF:Ag (µg/mL) | 13.4 (7.30–35.94) | 22.7 (15.8–28.4) | 0.224 |
| REAADS activity (%) × 21/VWF:Ag (µg/mL) | 0.193 (0.033–0.243) | 0.170 (0.120–0.241) | 0.627 |
| ADAMTS-13:Ag/VWF:Ag | 0.061 (0.027–0.150) | 0.038 (0.024–0.055) | 0.068 |
| LT (min) | 4.00 (3.33–4.70) | 3.67 (3.29–4.67) | 0.336 |
| PH (nM) | 184.0 (142.6–224.0) | 277.8 (206.7–299.8) | 0.006 |
| ttPeak (min) | 8.33 (7.00–9.30) | 6.56 5.67–7.83) | 0.003 |
| Including only samples obtained from trauma patients at 6 hours from TOI | Trauma (n=28 samples) | ||
| REAADS activity (%) | 92.0 (71.0–114.0) | 167.5 (108.0–312.5.0) | <0.001 |
| ADAMTS-13:Ag (µg/mL) | 1.00 (0.89–1.09) | 0.653 (0.531–0.821) | <0.001 |
| VWF:Ag (µg/mL) | 13.4 (7.30–35.9) | 18.5 (14.1–37.5) | 0.063 |
| REAADS activity (%) × 21/VWF:Ag (µg/mL) | 0.193 (0.033–0.243) | 0.147 (0.089–0.302) | 0.531 |
| ADAMTS-13:Ag/VWF:Ag | 0.061 (0.027–0.150) | 0.034 (0.013–0.044) | 0.006 |
| LT (min) | 4.00 (3.33–4.67) | 4.34 (3.61–4.97) | 0.715 |
| PH (nM) | 184.0 (142.6–224.0) | 226.1 (201.3–254.9) | 0.008 |
| ttPeak (min) | 8.33 (7.00–9.33) | 7.33 (6.34–8.49) | 0.035 |
Analyses were performed on all samples that were 0–2 hours from the TOI (top half of the table) and all samples that were 6 hours from the TOI (bottom half of the table), separately.
Plasma thrombin generation parameters (calibrated automated thrombogram) including LT, PH, and ttPeak are viewed against REAADS activity, ADAMSTS13:Ag level and VWF:Ag level for the same patient samples.
The ratio of REAADS activity (%) to VWF:Ag level is also presented and was calculated using the REAADS 21% value. Additionally, the ratio of ADAMTS-13:Ag level to VWF:Ag level was calculated for both trauma patients and volunteers and is presented.
*Kruskal-Wallis test for statistical significance. Results are presented as median values with IQR, with p<0.05 (bolded) are considered statistically significant.
ADAMTS-13:Ag, ADAMTS-13 antigen; LT, lagtime; PH, peak height; REAADS, Rapid Enzyme Assays for Autoimmune Diseases; TOI, time of injury; ttPeak, time to peak; VWF, von Willebrand factor; VWF:Ag, VWF antigen.